All functions

calcNearestStringDist()

Calculate distances to the nearest string

calculateFitnessScore()

Calculate fitness scores.

calculateRelativeFC()

Calculate logFCs relative to WT using edgeR

collapseMutantsBySimilarity() collapseMutantsByAA() collapseMutants()

Collapse mutants by similarity

digestFastqs()

Read, filter and digest sequences from fastq file(s).

generateQCReport()

Generate QC report

groupSimilarSequences()

Create a conversion table for collapsing similar sequences

linkMultipleVariants()

Process an experiment with multiple variable sequences

plotDistributions()

Plot distribution of observed values

plotFiltering()

Visualize the filtering procedure

plotMeanDiff()

Construct an MA (mean-difference) plot

plotPairs()

Make pairs plot of selected assay from a SummarizedExperiment object

plotTotals()

Plot the column totals of a selected assay

plotVolcano()

Construct a volcano plot

relabelMutPositions()

Relabel the positions of mutations in the designated ID

summarizeExperiment()

Summarize and collapse multiple mutational scanning experiments