MethylSeekR is a Bionductor/R package for the identification of unmethylated (UMRs) and low-methylated regions (LMRs) from whole-genome bisulfite-sequencing data. It can be downloaded from Bioconductor (

MethylSeekR segmentation results for 18 published human methylomes can be downloaded here: Identified segments (27.2MB)

Examples profiles showing the segmentation results for a representative subset of the data can be downloaded here:
Segmentation Examples (5.1MB)

The segmentation examples are multi-page pdf files for a representative set of methylomes. Each page shows the segmentation for a randomly chosen region. Each region displayed is broken up into 3 pairs of panels, where in each pair the same region is shown twice, once with raw methylation levels (top) and once with methylation levels smoothed over 3 consecutive CpGs (bottom). In both cases only CpGs with a coverage of at least 5 reads are shown. The raw data best illustrates the disordered nature of methylation levels in partially methylated domains(PMDs), whereas the smoothed methylation levels more clearly show UMRs and LMRs. In all figures, UMRs are shown as blue squares (placed at the middle of the identified segment), LMRs as red triangles (placed at the middle of the identified segment) and PMDs as green bars (extending over the entire PMD). The cut-off on methylation to determine UMRs and LMRs is shown as a grey dashed line.